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. 2013 Mar;51(3):1034-6.
doi: 10.1128/JCM.03369-12. Epub 2013 Jan 9.

A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Yanan Zhao  1 , Ruta Petraitiene, Thomas J Walsh, David S Perlin
Affiliations

A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Yanan Zhao et al. J Clin Microbiol. 2013 Mar.

Abstract

A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.

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Figures

Fig 1
Fig 1
Analytical sensitivity test (A) and linear quantification of E. rostratum in a real-time PCR (B). NTC, no-template control.
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