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. 2012:2012:429784.
doi: 10.1155/2012/429784. Epub 2012 Dec 12.

Prevalence of occult hepatitis C virus in egyptian patients with chronic lymphoproliferative disorders

Samar Samir Youssef  1 , Aml S Nasr, Taher El Zanaty, Rasha Sayed El Rawi, Mervat M Mattar
Affiliations

Prevalence of occult hepatitis C virus in egyptian patients with chronic lymphoproliferative disorders

Samar Samir Youssef et al. Hepat Res Treat. 2012.

Abstract

Background. Occult hepatitis C virus infection (OCI) was identified as a new form of Hepatitis C virus (HCV), characterized by undetectable HCV antibodies and HCV RNA in serum, while HCV RNA is detectable in liver and peripheral blood cells only. Aim. The aim of this study was to investigate the occurrence of OCI in Egyptian patients with lymphoproliferative disorders (LPDs) and to compare its prevalence with that of HCV in those patients. Subjects and Methods. The current study included 100 subjects, 50 of them were newly diagnosed cases having different lymphoproliferative disorders (patients group), and 50 were apparently healthy volunteers (controls group). HCV antibodies were detected by ELISA, HCV RNA was detected in serum and peripheral blood mononuclear cells (PBMCs) by reverse transcription polymerase chain reaction(RT-PCR), and HCV genotype was detected by INNO-LiPA. Results. OCI was detected in 20% of patients group, compared to only 4% OCI in controls group. HCV was detected in 26% of patients group with a slightly higher prevalence. There was a male predominance in both HCV and OCI. All HCV positive patients were genotype 4. Conclusion. Our data revealed occurrence of occult HCV infection in Egyptian LPD patients at a prevalence of 20% compared to 26% of HCV.

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Figures

Figure 1
Figure 1
Results of PCR amplification of HCV plus RNA strand in PBMC. PCR products of amplification of HCV plus strand from PBMCs of LPD patients (lanes 1, 2, 3, and 4). Lane N represents the negative control of the PCR. Lane 5 is the positive control of the PCR. Lane M is 100 Bp Mwt marker.
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