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. 2013 Feb;30(2):269-74.
doi: 10.1007/s10815-012-9905-9. Epub 2013 Jan 10.

A study of meiotic segregation in a fertile human population following ovarian stimulation with recombinant FSH-LH

Esther Velilla  1 , Silvia F Fernández, Jordi Suñol, Marisa López-Teijón
Affiliations

A study of meiotic segregation in a fertile human population following ovarian stimulation with recombinant FSH-LH

Esther Velilla et al. J Assist Reprod Genet. 2013 Feb.

Abstract

Objective: The aim of the study is to investigate the meiotic segregation in fresh eggs from anonymous egg donors and to analyze the baseline levels of aneuploidy in this population.

Results: The study includes the largest series of donor eggs so far studied: 203 eggs from donors aged between 20 and 31 years. No diagnosis was obtained in 10.8 % of cases (22/ 203). The biopsy of the first and second polar bodies was completed in a sequential manner on day 0 and day 1 of embryo development. Chromosomes 13, 16, 18, 21 and 22 are analyzed by means of the FISH test. The diagnosable fertilized eggs gave an aneuploidy rate of 19.1 % (31/162), with 83.8 % (26/31) of the errors produced during meiosis I, 12.9 % (4/31) produced during meiosis II, and 3.2 % (1/31) produced during both meiosis I and II. The premature division of sister chromatids is the main source of meiotic error during Meiosis I, resulting in the creation of oocyte aneuploidy.

Conclusions: FISH analysis of the first and second polar body in donor oocytes gave an aneuploidy rate of 19.1 %. This study shows the majority of errors occur during Meiosis I.

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Figures

Fig. 1
Fig. 1
Ovarian ultrasound. Ovarian stimulation: Scan control of follicular growing
Fig. 2
Fig. 2
Polar body hybridization. a: PB-1 first round. b: PB-1: second round with subtelomeric probes. c: PB-2: first round. d: PB-2: second round with subtelomeric probes
Fig. 3
Fig. 3
Meiosis I and II errors. Scheme of the different kinds of errors produced in Meiosis I and II
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