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. 1990 Mar;8(3):310-4.

Rapid and sensitive detection of DNA in Southern blots with chemiluminescence

I Bronstein  1 , J C Voyta, K G Lazzari, O Murphy, B Edwards, L J Kricka
Affiliations
  • PMID: 2331371

Rapid and sensitive detection of DNA in Southern blots with chemiluminescence

I Bronstein et al. Biotechniques. 1990 Mar.

Abstract

A new chemiluminescent Southern blot procedure offers molecular biologists a safe, ultrasensitive and rapid alternative to conventional 32P-based systems. This new DNA detection system, SOUTHERN-LIGHT, has been developed by Tropix, Inc. The luminescent signal is produced from a direct chemiluminescent substrate, disodium 3-(4-methoxyspiro[1,2-dioxetane-3-2'-tricyclo-[3.3.1.1 .3,7]decan]-4-yl) phenyl phosphate (AMPPD), which decomposes upon dephosphorylation with alkaline phosphatase. SOUTHERN-LIGHT is an ultrasensitive, rapid detection kit for use with membrane-bound DNA. It is the first test kit to incorporate AMPPD. It requires no specialized equipment and results can be conveniently imaged on instant film or x-ray film within 5-60 min of exposure.

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