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. 2013 Jun;115(6):654-7.
doi: 10.1016/j.jbiosc.2012.12.012. Epub 2013 Jan 11.

Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo  1 , Takashi Inaoka, Tsuyoshi Hachiya, Masayuki Miyake, Sumitaka Hase, Rikio Nakagawa, Hiromasa Hasegawa, Kazumi Funane, Yasubumi Sakakibara, Keitarou Kimura
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Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production

Yuji Kubo et al. J Biosci Bioeng. 2013 Jun.

Abstract

The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.

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