Enhancing the interface in silk-polypyrrole composites through chemical modification of silk fibroin

Abstract

To produce conductive, biocompatible, and mechanically robust materials for use in bioelectrical applications, we have developed a new strategy to selectively incorporate poly(pyrrole) (Ppy) into constructs made from silk fibroin. Here, we demonstrate that covalent attachment of negatively charged, hydrophilic sulfonic acid groups to the silk protein can selectively promote pyrrole absorption and polymerization within the modified films to form a conductive, interpenetrating network of Ppy and silk that is incapable of delamination. To further increase the conductivity and long-term stability of the Ppy network, a variety of small molecule sulfonic acid dopants were utilized and the properties of these silk-conducting polymer composites were monitored over time. The composites were evaluated using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), optical microscopy, energy-dispersive X-ray (EDX) spectroscopy, cyclic voltammetry, a 4-point resistivity probe and mechanical testing. In addition, the performance was evaluated following exposure to several biologically relevant enzymes. Using this strategy, we were able to produce mechanically robust polymer electrodes with stable electrochemical performance and sheet resistivities on the order of 1 × 10(2) Ω/sq (conductivity ∼1 S/cm).