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. 2013 Apr;51(4):1247-9.
doi: 10.1128/JCM.03316-12. Epub 2013 Jan 16.

Rapid and simultaneous detection of blaKPC and blaNDM by use of multiplex real-time PCR

Michael Milillo  1 , Yoon I Kwak, Erik Snesrud, Paige E Waterman, Emil Lesho, Patrick McGann
Affiliations

Rapid and simultaneous detection of blaKPC and blaNDM by use of multiplex real-time PCR

Michael Milillo et al. J Clin Microbiol. 2013 Apr.

Erratum in

Abstract

The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting blaKPC and blaNDM, two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with blaKPC- and blaNDM-carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.

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