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. 2012:1:80.
doi: 10.4103/2277-9175.102990. Epub 2012 Oct 31.

Study of promoter methylation pattern of 14-3-3 sigma gene in normal and cancerous tissue of breast: A potential biomarker for detection of breast cancer in patients

A Gheibi  1 , M Kazemi, A Baradaran, M Akbari, M Salehi
Affiliations

Study of promoter methylation pattern of 14-3-3 sigma gene in normal and cancerous tissue of breast: A potential biomarker for detection of breast cancer in patients

A Gheibi et al. Adv Biomed Res. 2012.

Abstract

Background: In recent years, DNA methylation as a main epigenetic modification in human cancer is found as a promising biomarker in early detection of breast cancer. Possible applications of numerous hypermethylated genes have been reported in diagnosis of breast cancer but there has been a little comprehensive study on the clinical usefulness of these genes in breast cancer. The aim of the present study was to investigate the promoter methylation status of 14-3-3 sigma gene with the goal of developing a diagnostic application in breast cancer.

Materials and methods: Totally 40 cases of cancerous and noncancerous tissues were studied. DNA was extracted from tissue samples, and promoter methylation pattern was determined by using methylation-specific polymerase chain reaction.

Results: Methylation pattern of 14-3-3 sigma promoter significantly differed between control and malignant breast tissues (P = 0.001), and there was no remarkable correlation between methylation and age (P > 0.05).

Conclusion: The relationship of promoter methylation of 14-3-3 sigma with development of breast cancer found in this study and confirmed the results of previous reports suggests that we can provide the foundation for possible application of 14-3-3 sigma as a potential biomarker for early detection and monitoring disease status.

Keywords: 14-3-3 Sigma; DNA methylation; early detection.

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Conflict of interest statement

Conflict of Interest: None declared

Figures

Figure 1
Figure 1
MSP analysis of T, cancerous and N normal breast tissue from one patient by U, unmethylated and M, methylated primers. C, control
Figure 2
Figure 2
Comparison of methylation pattern of 14-3-3 sigma promoter region in breast cancer and its adjacent normal tissues
See this image and copyright information in PMC

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