doi: 10.1007/978-1-62703-218-6_40.
Production of primary human CD4⁺ T cell lines and clones
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- PMID: 23329513
- PMCID: PMC4018300
- DOI: 10.1007/978-1-62703-218-6_40
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Production of primary human CD4⁺ T cell lines and clones
Methods Mol Biol.
2013.
Abstract
Tetramer staining of CD4(+) T cells is a valuable technique in immunology for detecting rare autoreactive T cells. Generating clones or cell lines from autoantigen tetramer-positive CD4(+) T cells allows further characterization and phenotyping of autoreactive cells.
Figures
Summary of methods 3.2 & 3.3, generating CD4+ T cell clones and cell lines. A: After primary stimulation of PBMC stain cultures with tetramer and antibody. B: Single cell sort CD4+ tetramer positive cells into a 96-well plate to generate a clone or bulk the cells into a sterile capped FACS tube and plate to generate a cell line. C: After stimulation and expansion test antigen specificity of cells with tetramer staining, as well as proliferation assays and cytokine staining (not shown).
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