doi: 10.1038/nmeth.2341.
Epub 2013 Jan 20.
A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets
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- PMID: 23334102
- DOI: 10.1038/nmeth.2341
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A biophysical miRNA-mRNA interaction model infers canonical and noncanonical targets
Nat Methods.
2013 Mar.
Abstract
We introduce a biophysical model of miRNA-target interaction and infer its parameters from Argonaute 2 cross-linking and immunoprecipitation data. We show that a substantial fraction of human miRNA target sites are noncanonical and that predicted target-site affinity correlates well with the extent of target destabilization. Our model provides a rigorous biophysical approach to miRNA target identification beyond ad hoc miRNA seed-based methods.
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