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. 2013 Feb 18;52(8):2364-7.
doi: 10.1002/anie.201208628. Epub 2013 Jan 22.

Liquid AP-UV-MALDI enables stable ion yields of multiply charged peptide and protein ions for sensitive analysis by mass spectrometry

Rainer Cramer  1 , Alexander Pirkl, Franz Hillenkamp, Klaus Dreisewerd
Affiliations
Free PMC article

Liquid AP-UV-MALDI enables stable ion yields of multiply charged peptide and protein ions for sensitive analysis by mass spectrometry

Rainer Cramer et al. Angew Chem Int Ed Engl. .
Free PMC article
No abstract available

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Figures

Figure 1
Figure 1
AP-UV-MALDI mass spectrum of MK-bradykinin (sequence: MKRPPGFSPFR), showing a) the m/z range 400–1600 and b) the m/z range 1200–1600. The matrix was the DHB-based liquid matrix containing approximately 20 % glycerol before volatile solvent evaporation.
Figure 2
Figure 2
AP-UV-MALDI CID MS/MS spectra of the a) doubly and b) triply protonated MK-bradykinin ions. The matrix was the DHB-based liquid matrix containing approximately 20 % glycerol before the evaporation of volatile solvents. The CID collision potentials were 35 V (a) and 20 V (b).
Figure 3
Figure 3
a) Total ion chromatogram (TIC) over a 30 min data acquisition using a liquid MALDI sample containing 500 fmol melittin. b) AP-UV-MALDI mass spectrum of the sum of all scans of the above acquisition. The matrix was the DHB-based liquid matrix containing approximately 20 % glycerol before evaporation of volatile solvents, and the laser repetition rate was 10 Hz.
Figure 4
Figure 4
a) AP-UV-MALDI mass spectrum of 5 pmol porcine insulin. The matrix was the DHB-based liquid matrix containing approximately 15 % glycerol before evaporation of volatile solvents. b) AP-UV-MALDI mass spectrum of 5 pmol horse heart myoglobin. The matrix was the CHCA-based 1-1-10 liquid matrix containing approximately 30 % glycerol before evaporation of volatile solvents. The pH value was above 7, as determined by a pH paper test strip. The detected [M+8 H]8+ and [M+9 H]9+ analyte ions can be attributed to the apo-form of myoglobin. In both spectra various polyethylene glycol (PEG) contaminant ions are also visible (possibly because of adverse storage conditions of the two samples over two years as fully diluted analyte solutions in plastic tubes).
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