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. 2013:7:19-24.
doi: 10.2147/DDDT.S39453. Epub 2013 Jan 9.

Impact of ghrelin on vitreous cytokine levels in an experimental uveitis model

Burak Turgut  1 , Fatih Cem Gül, Ferda Dağli, Nevin Ilhan, Metin Özgen
Affiliations

Impact of ghrelin on vitreous cytokine levels in an experimental uveitis model

Burak Turgut et al. Drug Des Devel Ther. 2013.

Abstract

Background: The purpose of this study was to investigate the effect of intraperitoneal ghrelin on vitreous levels of interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha (TNF-α) and to compare its effects with those of intraperitoneal infliximab in an experimental uveitis model.

Methods: Twenty-four male rats were assigned to four groups of six rats in each. All the rats, except for those in group 1 (controls), were injected intravitreally with concanavalin A to induce experimental uveitis. Rats in group 2 (sham) were not given any treatment after uveitis was induced. Rats in group 3 were given intraperitoneal infliximab 0.5 mg/100 mL on days 0, 1, 3, 5, and 7 following induction of uveitis on day 14 of the study. Rats in group 4 were given intraperitoneal ghrelin 10 ng/kg/day for 7 days following induction of uveitis. On day 21 of the study, enucleated globes were subjected to histopathologic examination. Vitreous levels of IL-1, IL-6, and TNF-α were measured by enzyme-linked immunosorbent assay.

Results: Vitreous levels of IL-1, IL-6, and TNF-α were significantly increased in the sham group relative to the control group (P < 0.05), but showed a significant decrease in the group treated with infliximab (P < 0.05). Cytokine levels also decreased in the ghrelin-treated group, but the decrease was not statistically significant (P > 0.05).

Conclusion: Ghrelin failed to decrease the IL-1, IL-6, and TNF-α levels that play a critical role in the pathogenesis of uveitis.

Keywords: ghrelin; infliximab; interleukin-1; interleukin-6; tumor necrosis factor-alpha; uveitis.

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References

    1. Franks WA, Limb GA, Stanford MR, et al. Cytokines in human intraocular inflammation. Curr Eye Res. 1992;11:187–191. - PubMed
    1. de Vos AF, Hoekzema R, Kijlstra A. Cytokines and uveitis: a review. Curr Eye Res. 1992;11:581–597. - PubMed
    1. de Vos AF, van Haren MA, Verhagen C, et al. Kinetics of intraocular tumor necrosis factor and interleukin-6 in endotoxin-induced uveitis in the rat. Invest Ophthalmol Vis Sci. 1994;35:1100–1106. - PubMed
    1. Ebert EC. Infliximab and the TNF-α system. Am J Physiol Gastrointest Liver Physiol. 2009;296:612–620. - PubMed
    1. Hosseini H, Safaei A, Khalili MR, et al. Intravitreal infliximab in experimental endotoxin-induced uveitis. Eur J Ophthalmol. 2009;19:818–823. - PubMed

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