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. 2013 Oct;13(8):1259-66.
doi: 10.2174/18715206113139990134.

Selection and characterization of human anti-MAGE-A1 scFv and immunotoxin

Hong Lin  1 , Yuan Mao, Da-Wei Zhang, Hu Li, Jin-Rong Qiu, Jin Zhu, Ren-Jie Chen
Affiliations

Selection and characterization of human anti-MAGE-A1 scFv and immunotoxin

Hong Lin et al. Anticancer Agents Med Chem. 2013 Oct.

Abstract

Melanoma-associated antigen (MAGE) is expressed on the surface of multiple tumor cell types and is a promising target of biotherapeutic drug delivery via the anti-MAGE-A1 antibody. In this study, a human single-chain variable fragment (scFv) antibody phage library was generated and applied to recombinant MAGE-A1-coated immunotubes by phage display technology. The soluble anti- MAGE-A1 scFv was expressed and purified by immobilized metal-chelated affinity chromatography (IMAC). The anti-MAGE-A1 scFv could bind native MAGE-A1 confirmed by enzyme-linked immunosorbent assay (ELISA), dot blot, and immunoprecipitation (IP) analysis. The immunotoxin was expressed and purified by IMAC successfully. The results indicated that the human anti-MAGE-A1 immunotoxin could provide a valuable drug for clinic cancer therapy.

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