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. 2012 Aug 7;1(8):e36.
doi: 10.1038/mtna.2012.31.

Rolling Circle Transcription of Tandem siRNA to Generate Spherulitic RNA Nanoparticles for Cell Entry

Peixuan Guo  1
Affiliations

Rolling Circle Transcription of Tandem siRNA to Generate Spherulitic RNA Nanoparticles for Cell Entry

Peixuan Guo. Mol Ther Nucleic Acids. .
No abstract available

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Figures

Figure 1
Figure 1
Schematic showing continuous synthesis of RNA by T7 RNA polymerase using rolling circle transcription.
Figure 2
Figure 2
Micrographs of the spherulitic RNA nanoparticles. (Upper left) Scanning electron microscopy (SEM) image of several spherulitic RNA nanoparticles clustered together, bar = 5 µm. (Upper right) Fluorescence microscopy image of spherulitic RNA nanoparticles stained with SYBR II dye, bar = 10 µm, and a higher magnification image in the inset, bar = 5 µm. (Lower left) Low magnification SEM image of spherulitic RNA nanoparticles, bar = 10 µm, and a higher magnification SEM image in the inset, bar = 500 nm. (Lower right) High magnification SEM image showing the sheet-like substructure of the spherulitic RNA nanoparticles, bar = 500 nm. Figures reproduced with permission from ref. , © 2012 NPG.
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