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. 2012 Dec 21;18(1):114-27.
doi: 10.3390/molecules18010114.

Chemical constituents of three Allium species from Romania

Laurian Vlase  1 , Marcel Parvu, Elena Alina Parvu, Anca Toiu
Affiliations

Chemical constituents of three Allium species from Romania

Laurian Vlase et al. Molecules. .

Abstract

The aim of this work was to study the chemical composition of Allium obliquum L., A. senescens L. subsp. montanum (Fries) Holub, and A. schoenoprasum L. subsp. schoenoprasum. Sulphur-containing compounds analysis was performed by an LC-MS method, the identification and quantification of polyphenolic compounds through a HPLC-UV-MS method, and the presence of five sterols was simultaneously assessed by HPLC-MS-MS. Alliin was identified only in A. obliquum and A. senescens subsp. montanum extracts, whilst allicin was present in all extracts, with higher amounts in A. schoenoprasum and A. obliquum. The pattern of phenol carboxylic acids shows the presence of p-coumaric and ferulic acids in all species. Isoquercitrin was identified in A. obliquum and A. schoenoprasum, and rutin in A. senescens subsp. montanum and A. schoenoprasum. Luteolin and apigenin were identified only in A. obliquum. All three species contain glycosides of kaempferol and quercetol. β-Sitosterol and campesterol were identified in all species. The results obtained showed significant differences in the composition of the three Allium species.

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Figures

Figure 1
Figure 1
HPLC chromatogram of a non-hydrolysed sample of A. senescens subsp. montanum (A2 N).
Figure 2
Figure 2
HPLC chromatogram of a hydrolysed sample of A. senescens subsp. montanum (A2 H).
Figure 3
Figure 3
HPLC chromatogram of hydrolysed sample of A. schoenoprasum L. subsp. schoenoprasum (A3 H).
Figure 4
Figure 4
(a) The LC-MS chromatogram of allicin from A. schoenoprasum extract; (b) MS-MS spectra of allicin.
Figure 5
Figure 5
(a) The chromatogram of A. obliquum extract; (b) MS-MS spectra of alliin.
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