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. 2013 Jan 17;18(1):1214-26.
doi: 10.3390/molecules18011214.

Protective effect of ginsenoside Rb1 against lung injury induced by intestinal ischemia-reperfusion in rats

Jin Wang  1 , Lifen Qiao, Shusheng Li, Guangtian Yang
Affiliations

Protective effect of ginsenoside Rb1 against lung injury induced by intestinal ischemia-reperfusion in rats

Jin Wang et al. Molecules. .

Abstract

Intestinal ischemia-reperfusion (I/R) is a critical event in the pathogenesis of multiple organ dysfunction syndromes (MODS). The lungs are some of the most vulnerable organs that are impacted by intestinal I/R. The aim of this study is to investigate whether ginsenoside Rb1 can ameliorate remote lung injury induced by intestinal I/R. Adult male Wistar rats were randomly divided into four groups: (1) a control, sham-operated group (sham group); (2) an intestinal I/R group subjected to 1 h intestinal ischemia and 2 h reperfusion (I/R group); (3) a group treated with 20 mg/kg ginsenoside Rb1 before reperfusion (Rb1-20 group); and (4) a group treated with 40 mg/kg ginsenoside Rb1 before reperfusion (Rb1-40 group). Intestinal and lung histology was observed. The malondialdehyde (MDA) levels in intestinal tissues were measured. Myeloperoxidase (MPO), TNF-α, MDA levels, wet/dry weight ratio and immunohistochemical expression of intracellular adhesion molecule-1 (ICAM-1) in lung tissues were assayed. In addition, a western blot of lung NF-kB was performed. Results indicated that intestinal I/R induced intestinal and lung injury, which was characterized by increase of MDA levels and pathological scores in intestinal tissues and MPO, TNF-α , MDA levels, wet/dry weight ratio and ICAM-1, NF-kB expression in the lung tissues. Ginsenoside Rb1 (20, 40 mg/kg) ameliorated intestinal and lung injury, decreased MPO, TNF-α, MDA levels, wet/dry weight ratio, ICAM-1 and NF-kB expression in lung tissues. In conclusion, ginsenoside Rb1 ameliorated the lung injuries by decreasing the NF-kB activation-induced inflammatory response.

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Figures

Figure 1
Figure 1
The molecular structure of Rb1.
Figure 2
Figure 2
(A) Effects of Rb1 on intestinal injury after intestinal I/R. (HE ×400); (B) Intestinal pathological scores of different groups.
Figure 3
Figure 3
(A) Effects of Rb1 on lung injury after intestinal I/R. (HE ×400); (B) Lung pathological scores of different groups.
Figure 4
Figure 4
Effects of Rb1 on MDA levels of intestinal and lung tissues.
Figure 5
Figure 5
Effects of Rb1 on lung wet weight to dry weight ratio.
Figure 6
Figure 6
Effects of Rb1 on TNF-α levels of lung.
Figure 7
Figure 7
Effects of Rb1 on MPO levels of lung.
Figure 8
Figure 8
Effects of Rb1 on ICAM-1 expression of lung tissue after intestinal I/R (×200).
Figure 9
Figure 9
Effects of Rb1 on NF-kB activation of lung tissue after intestinal I/R.
See this image and copyright information in PMC

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