doi: 10.1128/JCM.03003-12.
Epub 2013 Jan 23.
Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens
Affiliations
- PMID: 23345287
- PMCID: PMC3666792
- DOI: 10.1128/JCM.03003-12
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Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens
J Clin Microbiol.
2013 Apr.
Abstract
The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability.
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