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. 2013 Apr;51(4):1260-2.
doi: 10.1128/JCM.03003-12. Epub 2013 Jan 23.

Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens

Gavin A Cloherty  1 , James Rhoads, Thomas P Young, Neil T Parkin, Vera Holzmayer, Lilly Yuen, Carolyn Mullen
Affiliations

Sequence conservation of the region targeted by the Abbott RealTime HBV viral load assay in clinical specimens

Gavin A Cloherty et al. J Clin Microbiol. 2013 Apr.

Abstract

The Abbott RealTime HBV assay targets the N-terminal region of the S gene. Here we analyzed the sequence variability of the assay target region from >2,100 clinical specimens. Thermodynamic modeling of the percentage of bound primer/probe at the assay annealing temperature was performed to assess the potential effect of sequence variability.

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