Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Apr;51(4):1250-2.
doi: 10.1128/JCM.02914-12. Epub 2013 Jan 23.

Microplate alamarBlue assay for Paracoccidioides susceptibility testing

A C A de Paula e Silva  1 , H C Oliveira, J F Silva, F Sangalli-Leite, L Scorzoni, A M Fusco-Almeida, M J S Mendes-Giannini
Affiliations

Microplate alamarBlue assay for Paracoccidioides susceptibility testing

A C A de Paula e Silva et al. J Clin Microbiol. 2013 Apr.

Abstract

CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs.

PubMed Disclaimer

Figures

Fig 1
Fig 1
MMA and MMI tests developed with P. brasiliensis 18 against AMB and ITZ. The MIC values were determined after the addition of alamarBlue.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bagagli E, Bosco SM, Theodoro RC, Franco M. 2006. Phylogenetic and evolutionary aspects of Paracoccidioides brasiliensis reveal a long coexistence with animal hosts that explain several biological features of the pathogen. Infect. Genet. Evol. 6:344–351 - PubMed
    1. Bagagli E, Theodoro RC, Bosco SM, McEwen JG. 2008. Paracoccidioides brasiliensis: phylogenetic and ecological aspects. Mycopathologia 165:197–207 - PubMed
    1. Matute DR, Sepulveda VE, Quesada LM, Goldman GH, Taylor JW, Restrepo A, McEwen JG. 2006. Microsatellite analysis of three phylogenetic species of Paracoccidioides brasiliensis. J. Clin. Microbiol. 44:2153–2157 - PMC - PubMed
    1. Teixeira MM, Theodoro RC, de Carvalho MJ, Fernandes L, Paes HC, Hahn RC, Mendoza L, Bagagli E, San-Blas G, Felipe MS. 2009. Phylogenetic analysis reveals a high level of speciation in the Paracoccidioides genus. Mol. Phylogenet. Evol. 52:273–283 - PubMed
    1. Coutinho ZF, Silva D, Lazera M, Petri V, Oliveira RM, Sabroza PC, Wanke B. 2002. Paracoccidioidomycosis mortality in Brazil (1980-1995). Cad. Saude Publica 18:1441–1454 - PubMed

Publication types

MeSH terms

LinkOut - more resources