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. 2013 Jan 5;21(1):5.
doi: 10.1186/2008-2231-21-5.

Isolation and characterization of exopolysaccharide with immunomodulatory activity from fermentation broth of Morchella conica

Chao-An Su  1 , Xiao-Yan Xu, De-Yun Liu, Ming Wu, Fan-Qing Zeng, Meng-Yao Zeng, Wei Wei, Nan Jiang, Xia Luo
Affiliations

Isolation and characterization of exopolysaccharide with immunomodulatory activity from fermentation broth of Morchella conica

Chao-An Su et al. Daru. .

Abstract

BACKGROUND AND THE PURPOSE OF THIS STUDY: Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. In vitro and in vivo studies suggest that certain polysaccharides affect immune system function. Morchella conica (M. conica) is a species of rare edible mushroom whose multiple medicinal functions have been proven. Thus, the objective of this study is to isolate and characterize of exopolysaccharide from submerged mycelial culture of M. conica, and to evaluate its immunomodulatory activity.

Methods: A water-soluble Morchella conica Polysaccharides (MCP) were extracted and isolated from the fermentation broth of M. conica through a combination of DEAE-cellulose and Sephacryl S-300 HR chromatograph. NMR and IR spectroscopy has played a developing role in identification of polysaccharide with different structure and composition from fungal and plant sources, as well as complex glycosaminoglycans of animal origin. Thus, NMR and IR spectroscopy were used to analyze the chemical structure and composition of the isolated polysaccharide. Moreover, the polysaccharide was tested for its immunomodulatory activity at different concentrations using in vitro model.

Results: The results showed that MCP may significantly modulate nitric oxide production in macrophages, and promote splenocytes proliferation. Analysis from HPLC, infrared spectra and nuclear magnetic resonance spectroscopy showed that MCP was a homogeneous mannan with an average molecular weight of approximately 81.2 kDa. The glycosidic bond links is →6)-α-D-Man p-(1→.

Conclusion: The results suggested that the extracted MCP may modulate nitric oxide production in macrophages and promote splenocytes proliferation, and it may act as a potent immunomodulatory agent.

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Figures

Figure 1
Figure 1
Elution pattern of the crude polysaccharide produced from submerged mycelial culture of M.conica on DEAE-Cellulose 52 anion-exchange chromatography column (2.6 × 50 cm, Whatmann). The four peaks of P1, P2, P3 and P4 were eluted with a linear gradient of NaCl from 0 mM to 500 mM in 20 mM Tris–HCl (pH 8.0), and the activity were also measured.
Figure 2
Figure 2
IR analysis of M. conica polysaccharides (MCP).
Figure 3
Figure 3
Effects of polysaccharides of MCP and LPS on NO production in RAW264.7 cells. Data represent mean values ± SD (n = 3).
Figure 4
Figure 4
Effects of different concentrations of MCP, Con A- and LPS-stimulated splenocyte proliferation in vitro. Data represent mean values ± SD (n = 3).
See this image and copyright information in PMC

References

    1. Wasser SP. Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Appl Microbiol Biotechnol. 2011;89:1323–1332. doi: 10.1007/s00253-010-3067-4. - DOI - PubMed
    1. Stachowiak B, Reguła J. Health-promoting potential of edible macromycetes under special consideration of polysaccharides: a review. Eur Food Res Technol. 2012;234:369–380. doi: 10.1007/s00217-011-1656-9. - DOI
    1. Borchers AT, Krishnamurthy A, Keen CL, Meyers FJ, Gershwin ME. The immunobiology of mushrooms. Exp Biol Med. 2008;233:259–276. doi: 10.3181/0708-MR-227. - DOI - PubMed
    1. Schepetkin IA, Xie G, Kirpotina LN, Klein RA, Jutila MA, Quinn MT. Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha. Int Immunopharmacol. 2008;8:1455–1466. doi: 10.1016/j.intimp.2008.06.003. - DOI - PMC - PubMed
    1. Cheng XQ, Li H, Yue XL, Xie JY, Zhang YY, Di HY, Chen DF. Macrophage immunomodulatory activity of the polysaccharides from the roots of Bupleurum smithii var. parvifolium. Ethnopharmacol. 2010;130:363–368. doi: 10.1016/j.jep.2010.05.019. - DOI - PubMed