Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Mar 26;262(2):323-6.
doi: 10.1016/0014-5793(90)80219-9.

Structure-function analysis of human interleukin-6. Evidence for the involvement of the carboxy-terminus in function

A Krüttgen  1 , S Rose-John, C Möller, B Wroblowski, A Wollmer, J Müllberg, T Hirano, T Kishimoto, P C Heinrich
Affiliations
Free article

Structure-function analysis of human interleukin-6. Evidence for the involvement of the carboxy-terminus in function

A Krüttgen et al. FEBS Lett. .
Free article

Abstract

C-terminally deleted analogs of human interleukin-6 (IL-6) have been constructed at the cDNA level, and after cell-free transcription and translation their biological activity was analyzed. Removal of only 4 amino acids resulted in complete loss of biological activity as determined by the B9 cell proliferation assay. Secondary structure prediction of human IL-6 resulted in 58% helix, 14% beta-structure, and 28% turn and coil (average of 3 independent methods). The circular dichroism of recombinant human IL-6 was measured in the near and far UV. Evaluation of the latter in terms of secondary structures gave 67% helix, 15% beta-structure, and 18% turn and coil.

PubMed Disclaimer

Publication types

LinkOut - more resources