Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jan 24:6:198.
doi: 10.3389/fnins.2012.00198. eCollection 2012.

Medulloblastoma or not? Crucial role in tumorigenesis of the timing of migration of cerebellar granule precursor cells, regulated by Nos2 and Tis21

Stefano Farioli-Vecchioli  1 , Laura Micheli, Luca Leonardi, Manuela Ceccarelli, Sebastiano Cavallaro, Felice Tirone
Affiliations

Medulloblastoma or not? Crucial role in tumorigenesis of the timing of migration of cerebellar granule precursor cells, regulated by Nos2 and Tis21

Stefano Farioli-Vecchioli et al. Front Neurosci. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Proposed models of plasticity and transformation of cerebellar granule precursor cells (GCPs) in response to Math1, Tis21, Nos2, Cxcl3, or Gap43. (A) Hypothetical model for the ambivalent role of Math1 (Atoh1) in tumorigenesis and differentiation; in a proliferative (Shh) environment Math1 favors tumorigenesis, while when GCPs are exposed to a differentiative signal (Tis21), Math1 may have a prodifferentiative action. Math1 and Id3 were not selected among the 344 genes whose expression is modified by Tis21-knockout in the report by Farioli-Vecchioli et al. (2012a), because of the stringent cut-off imposed on the statistical analysis. See Figure A1 for analysis of Math 1 and Id3 mRNA levels in GCPs of Tis21-null mice. (B) Epigenetically-regulated genes among the 344 Tis21-regulated genes, either Tis21-knockout-specific (pairwise comparison of Tis21KO vs. Tis21WT) or medulloblastoma-specific (pairwise comparison of Ptch1+/−/Tis21KO vs. Ptch1+/−/Tis21WT); see (Farioli-Vecchioli et al., 2012a) and Figure A1 for enrichment analysis. (C) Model of plasticity and transformation of GCPs, involving Cxcl3- and Gap43-induced migration (see text). EGL, external granular layer (surface of cerebellum); ML, molecular layer; IGL, internal granular layer.
Figure A1
Figure A1
(A) Math1 and Id3 mRNA expression values by real time PCR analysis performed in GCPs from 7-days-old mice of the four genotypes indicated. Math1 mRNA levels are reduced in GCPs of Tis21 knockout mice, either in a Patched1 wild-type background (Tis21KO vs. Tis21WT) or in a Patched1 heterozygous background (Ptch1+/−/Tis21KO vs. Ptch1+/−/Tis21WT). Mean ± SEM fold increases are from three independent experiments. TBP was used to normalize data. *P < 0.05, or **P < 0.01, Student's t-test. (B) Enrichment analysis to identify epigenetically-regulated genes among the Tis21-regulated genes. Among the 344 Tis21-regulated genes, either Tis21-knockout-specific (pairwise comparison of Tis21KO vs. Tis21WT) or medulloblastoma-specific (pairwise comparison of Ptch1+/−/Tis21KO vs. Ptch1+/−/Tis21WT; see Farioli-Vecchioli et al., 2012a) genes were selected whose product interacts with histone modifying factors [i.e., arginine N-methyltransferase 1 (Prmt1), or histone deacetylases] or whose expression is significantly affected by inhibitors of DNA methylases or histone deacetylases. Enrichment P values were obtained with Fisher's exact test.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Bossuyt W., Kazanjian A., De Geest N., Van Kelst S., De Hertogh G., Geboes K., et al. (2009). Atonal homolog 1 is a tumor suppressor gene. PLoS Biol. 7:e39 10.1371/journal.pbio.1000039 - DOI - PMC - PubMed
    1. Canzoniere D., Farioli-Vecchioli S., Conti F., Ciotti M. T., Tata A. M., Augusti-Tocco G., et al. (2004). Dual control of neurogenesis by PC3 through cell cycle inhibition and induction of Math1. J. Neurosci. 24, 3355–3369 10.1523/JNEUROSCI.3860-03.2004 - DOI - PMC - PubMed
    1. Choi Y., Borghesani P. R., Chan J. A., Segal R. A. (2005). Migration from a mitogenic niche promotes cell-cycle exit. J. Neurosci. 25, 10437–10445 10.1523/JNEUROSCI.1559-05.2005 - DOI - PMC - PubMed
    1. Farioli-Vecchioli S., Cinà I., Ceccarelli M., Micheli L., Leonardi L., Ciotti M. T., et al. (2012a). Tis21 knockout enhances the frequency of medulloblastoma in Patched1 heterozygous mice by inhibiting the Cxcl3-dependent migration of cerebellar neurons. J. Neurosci. 32, 15547–15564 10.1523/JNEUROSCI.0412-12.2012 - DOI - PMC - PubMed
    1. Farioli-Vecchioli S., Micheli L., Saraulli D., Ceccarelli M., Cannas S., Scardigli R., et al. (2012b). Btg1 is required to maintain the pool of stem and progenitor cells of the dentate gyrus and subventricular zone. Front. Neurosci. 6:124 10.3389/fnins.2012.00124 - DOI - PMC - PubMed

LinkOut - more resources