Outbreak of NDM-1-producing Klebsiella pneumoniae in a neonatal unit in Colombia

Abstract

Six multiresistant, NDM-1-producing Klebsiella pneumoniae strains were recovered from an outbreak that affected six neonatal patients in a Colombian hospital. Molecular analysis showed that all of the isolates harbored the blaNDM-1, qnrA, and intI1 genes and were clonally related. Multilocus sequence typing showed that the isolates belonged to a new sequence type (ST1043) that was different from the sequence types that had previously been reported. This is the first report of NDM-1-producing isolates in South America.

Fig 1

Comparative map of the genetic elements surrounding the bla_NDM-1 gene found in the 16Kpn1 isolate and Acinetobacter pittii strain ABCA95 plasmid pABCA95, Acinetobacter lwoffii strain WJ10621 plasmid pNDM-BJ01, Acinetobacter haemolyticus strain ABC7926 plasmid pABC7926, A. baumannii strain 161/07, A. baumannii strain JH, E. coli strain N10-2337 plasmid pNDM102337, E. coli strain DVR22, Providencia stuartii plasmid pMR0211, K. pneumoniae strain Kp7 plasmid pNDM-KN, and K. pneumoniae plasmid pTR3 (GenBank accession numbers JQ739157.1, JQ001791.1, JQ080305.1, HQ857107.1, JN872329.1, JF714412.1, JF922606.1, JN687470.1, JN157804.1, and JQ349086.2, respectively).