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. 2013 Mar;31(3):230-2.
doi: 10.1038/nbt.2507. Epub 2013 Jan 29.

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Seung Woo Cho  1 , Sojung Kim, Jong Min Kim, Jin-Soo Kim
Affiliations

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Seung Woo Cho et al. Nat Biotechnol. 2013 Mar.

Abstract

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

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Comment in

  • RNA-guided gene editing.
    de Souza N. de Souza N. Nat Methods. 2013 Mar;10(3):189. doi: 10.1038/nmeth.2389. Nat Methods. 2013. PMID: 23565557 No abstract available.

References

    1. Nat Biotechnol. 2013 Mar;31(3):227-9 - PubMed
    1. Science. 2011 May 13;332(6031):784-5, 787-9 - PubMed
    1. Nature. 2012 Feb 15;482(7385):331-8 - PubMed
    1. Nat Biotechnol. 2008 Jul;26(7):808-16 - PubMed
    1. Genome Res. 2012 Jul;22(7):1327-33 - PubMed

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