Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990;76(4):289-93.
doi: 10.1007/BF00928181.

A new approach to the phylogeny of Leishmania: species specificity of glycoconjugate ligands for promastigote internalization into murine macrophages

C B Palatnik  1 , J O Previato, L Mendonça-Previato, R Borojevic
Affiliations

A new approach to the phylogeny of Leishmania: species specificity of glycoconjugate ligands for promastigote internalization into murine macrophages

C B Palatnik et al. Parasitol Res. 1990.

Abstract

Two Leishmania donovani glycoconjugate ligands for the internalization receptor on BALB/c peritoneal macrophages [fucose-mannose ligand (FML) and phosphate mannogalactan ligand (PMGL)] were shown to be species-specific in a comparative phagocytosis-inhibition test. Promastigotes of L. donovani Sudan (LD1S), L. infantum, L. d. donovani, L. major (Jericho and Sudan), L. tropica, L. chagasi, L. mexicana venezuelensis, L. m. mexicana, L. m. amazonensis, L. m. pifanoi, L. m. garnhami, L. braziliensis braziliensis, L. m. amazonensis (Josefa), L. enrietti or L. adleri were incubated with macrophages in the presence of 10 micrograms/ml FML and PMGL purified from L. donovani (LD1S). Parasite internalization was determined and compared with that obtained in control experiments. Specific inhibition of phagocytosis ranged from 83% (L. donovani LD1S) to 7% (L. m. amazonensis). We could distinguish groups of Leishmania consistently with their geographic distribution and the clinical aspects of the disease. Analogous experiments with L. m. amazonensis glycoconjugates showed reciprocal results, with inhibition ranging from 76% (L. m. amazonensis) to 8% (L. donovani LD1S). L. chagasi remained separated from the Old World kala-azar agents. Possible phylogenetic implications of these observations are discussed.

PubMed Disclaimer

References

    1. Exp Parasitol. 1966 Feb;18(1):124-93 - PubMed
    1. Isr J Med Sci. 1972 Jul;8(7):932-42 - PubMed
    1. Comp Biochem Physiol B. 1987;86(3):593-9 - PubMed
    1. World Health Organ Tech Rep Ser. 1984;701:1-140 - PubMed
    1. Infect Immun. 1989 Mar;57(3):754-63 - PubMed

Publication types