doi: 10.1016/0165-2427(90)90019-o.
The characterisation of equine interleukin-1
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- PMID: 2336790
- DOI: 10.1016/0165-2427(90)90019-o
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The characterisation of equine interleukin-1
Vet Immunol Immunopathol.
1990 Feb.
Abstract
Equine interleukin-1 has been produced from peripheral blood monocytes by stimulation with E. coli lipopolysaccharide. Sephacryl S200 gel filtration revealed a molecular weight of 17-18 kD. Chromatofocusing of the 17-18 kD peak identified four active fractions. Two major peaks were detected at pH 6.7 and pH 7, with smaller peaks at pH 6.3 and pH 5.9. The pI 7 molecule is probably the equine form of IL-1 beta.
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