Impact of single nucleotide polymorphism in tumor necrosis factor-α gene 308G/A in Egyptian asthmatic children and wheezing infants

Abstract

Bronchial asthma is a common disease with multiple determinants that include genetic variation. Although tumor necrosis factor alpha (TNF-α) is a major pro-inflammatory cytokine, the functions of genetic polymorphisms in this cytokine has not been thoroughly examined in the context of asthma pathology. Therefore, we aimed to investigate whether single nucleotide polymorphism (SNP) in TNF-α is associated with asthma and wheezing and whether the association is related to the severity of the disease and other epidemiological factors. Frequencies of TNF-α-308G/A polymorphism were compared in 100 asthmatic children, 100 wheezy infants and 100 age and gender matched controls. Genotype frequencies for TNF-α-308G/A were significantly higher in asthmatic children (60%) and wheezy infants (68%) than the control group (30%). Higher serum levels of TNF-α were observed in genotypes G/A and G/G of asthmatic children and wheezy infants than in controls. No association was found between the G/A polymorphism and the severity of the disease, the total eosinophil count and IgE levels in both groups. We can conclude that genetic variation in TNF-α-308G/A may contribute to childhood asthma and wheezing. These findings could be helpful for future early intervention studies which may have a potential impact on family counseling and management.