Stem cells: insights into the secretome

Abstract

Stem cells have been considered as possible therapeutic vehicles for different health related problems such as cardiovascular and neurodegenerative diseases and cancer. Secreted molecules are key mediators in cell-cell interactions and influence the cross talk with the surrounding tissues. There is strong evidence supporting that crucial cellular functions such as proliferation, differentiation, communication and migration are strictly regulated from the cell secretome. The investigation of stem cell secretome is accumulating continuously increasing interest given the potential use of these cells in regenerative medicine. The scope of the review is to report the main findings from the investigation of stem cell secretome by the use of contemporary proteomics methods and discuss the current status of research in the field. This article is part of a Special Issue entitled: An Updated Secretome.

Keywords: 2 Dimensional Differential Gel Electrophoresis; 2 Dimensional Electrophoresis; 2D-DIGE; 2DE; AF-MSCs; AHF; AT tau; Acute Hepatic Failure; Alzheimer's truncated tau; Amniotic Fluid derived Mesenchymal Stem Cells; BMSCs; Bone Marrow derived Mesenchymal Stem Cells; CKD; CM; CSCs; Cancer Stem cells; Chronic Kidney Disease; Conditioned Medium; Cyr 61; Cysteine rich protein 61; GRO; GSCs; GeLC-MS/MS; Glioblastoma Stem like Cells; Growth Related Oncogene; HDGF; HPL; HUVEC; Hepatic Progenitor-Like cells; Hepatoma Derived Growth Factor; Human Umbilical Vein Endothelial Cells; IE-MS; IFN γ; IL-8; IP-10; Interferon gamma; Interferon gamma-induced Protein 10; Interleukin 8; Iterative Exclusion-Mass Spectrometry; Left-right determination factor 1; Lefty 1; MCP-1; MEF; MIAMI; MS; Marrow Isolated Adult Multilineage Inducible cells; Mass Spectrometry; Monocyte Chemotactic Protein 1; Mouse Embryonic Fibroblast; Murine Mesenchymal Stem Cells; NSCs; Neural Stem Cells; OSM; Osteogenic Medium; Ovx; PAI-1; Plasminogen Activator Inhibitor 1; Proteomics; SDF-1; SDS PAGE combined with Liquid Chromatography Tandem Mass Spectrometry; SMOC1; SPARC related Modular Calcium-binding protein 1; Secretome; Stem cells; Stromal cell-Derived Factor 1; TNF-α; Tumor Necrosis Factor alpha; VEGF; Vascular Endothelial Growth Factor; hAFSCs; hAMSCs; hASCs; hBMSCs; hESCs; hMADS; human Adipose tissue-derived Mesenchymal Stem Cells; human Adipose tissue-derived Stem Cells; human Amniotic Fluid Stem Cells; human Bone Marrow-derived Mesenchymal Stem Cells; human Embryonic Stem Cells; human Multipotent Adipose-Derived Stem cells; mESCs; mMSCs; murine Embryonic Stem Cells; ovariectomized.