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. 2013 Mar;10(3):249-52.
doi: 10.1038/nmeth.2360. Epub 2013 Feb 3.

Optogenetic protein clustering and signaling activation in mammalian cells

Lukasz J Bugaj  1 , Atri T Choksi, Colin K Mesuda, Ravi S Kane, David V Schaffer
Affiliations

Optogenetic protein clustering and signaling activation in mammalian cells

Lukasz J Bugaj et al. Nat Methods. 2013 Mar.

Abstract

We report an optogenetic method based on Arabidopsis thaliana cryptochrome 2 for rapid and reversible protein oligomerization in response to blue light. We demonstrated its utility by photoactivating the β-catenin pathway, achieving a transcriptional response higher than that obtained with the natural ligand Wnt3a. We also demonstrated the modularity of this approach by photoactivating RhoA with high spatiotemporal resolution, thereby suggesting a previously unknown mode of activation for this Rho GTPase.

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