Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Mar;10(3):256-8.
doi: 10.1038/nmeth.2362. Epub 2013 Feb 3.

Generating parabiotic zebrafish embryos for cell migration and homing studies

Doris Lou Demy  1 , Zachary Ranta, Jean-Michel Giorgi, Magali Gonzalez, Philippe Herbomel, Karima Kissa
Affiliations

Generating parabiotic zebrafish embryos for cell migration and homing studies

Doris Lou Demy et al. Nat Methods. 2013 Mar.

Abstract

Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds.

PubMed Disclaimer

References

    1. Nature. 2010 Mar 4;464(7285):112-5 - PubMed
    1. Folia Biol (Praha). 1979;25(5):293-5 - PubMed
    1. Dev Cell. 2005 Mar;8(3):389-400 - PubMed
    1. Dev Biol. 2001 Oct 15;238(2):274-88 - PubMed
    1. Blood. 2004 Sep 1;104(5):1291-7 - PubMed

Publication types

LinkOut - more resources