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. 2013 Jun;158(6):1397-401.
doi: 10.1007/s00705-013-1608-8. Epub 2013 Feb 5.

Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China

Jianfei Chen  1 , Xiaozhen Liu, Hongwu Lang, Zhongtian Wang, Da Shi, Hongyan Shi, Xin Zhang, Li Feng
Affiliations

Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China

Jianfei Chen et al. Arch Virol. 2013 Jun.

Abstract

Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006-2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.

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Figures

Fig. 1
Fig. 1
Phylogenetic analysis of the nucleotide sequences of N genes of PEDV field strains. The tree was constructed based on the neighbor-joining method using the MEGA 5.05 software. The scale bar indicates the branch lengths for 0.2 % nucleotide differences. The Chinese PEDV strains are marked with triangles
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