Localization of the adenosine deaminase, transferrin, and UMP synthetase genes on Chinese hamster chromosomes 4 and 6 by in situ hybridization

Abstract

Careful analysis of G-band patterns in various rodent families allows identification of homology and thus accurate prediction of gene map positions. However, conclusions based on the synteny of genes without a careful study of chromosome evolution and G-band homology can be misleading. We tested these generalizations by means of G-band analysis and in situ hybridization with three genes in Chinese hamster (Cricetulus griseus, CGR) chromosomes. The location of the adenosine deaminase gene, previously mapped by somatic cell hybrid panels, was confirmed and further sublocalized on CGR 6q1. Although transferrin and uridine monophosphate synthetase are localized to adjacent bands on human chromosome 3 (3q21 and 3q13, respectively), we report that these genes are widely separated on CGR 4q2 and 4p2, respectively.