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. 1978 Dec 1;175(3):1139-41.
doi: 10.1042/bj1751139.

A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues

H P Bennett, A M Hudson, L Kelly, C McMartin, G E Purdon

A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues

H P Bennett et al. Biochem J. .

Abstract

Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.

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