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. 2013 Feb 11:12:32.
doi: 10.1186/1475-2840-12-32.

Intense exercise training is not effective to restore the endothelial NO-dependent relaxation in STZ-diabetic rat aorta

Mohamed Sami Zguira  1 , Sophie Vincent, Solène Le Douairon Lahaye, Ludivine Malarde, Zouhair Tabka, Bernard Saïag
Affiliations

Intense exercise training is not effective to restore the endothelial NO-dependent relaxation in STZ-diabetic rat aorta

Mohamed Sami Zguira et al. Cardiovasc Diabetol. .

Abstract

Background: The aim of this study was to examine the effects of intense physical training on vascular function in streptozotocin-diabetic rats. We focused on the endothelium-dependent relaxation (EDR) induced by acetylcholine (ACh) and stable ADP adenosine-5'- O - (2-thiodiphosphate) (ADPβS).

Methods: Control or diabetic male Wistar rats (n=44) were randomly assigned to sedentary or trained groups. The training program consisted in a regular period of running on a treadmill during 8 weeks (10° incline and up to 25 m/min, 60 min/day). The reactivity of isolated thoracic aorta rings of healthy, diabetic and/or trained has been tested.

Results: ACh and ADPβS-induced EDR were observed in phenylephrine (PE) pre-contracted vessels. As compared to sedentary control group, diabetic rats showed an increase in PE-induced contraction and a decrease in ACh and ADPβS-induced EDR (p<0.05). Moreover, there were no increase in ACh and ADPβS-induced EDR in diabetic rats. N-Nitro-L-Arginine Methyl Ester inhibited the nitric oxide synthase in diabetic and control rats, thereby resulting in a strong inhibition of the EDR induced by ACh and ADPβS (10-6 M).

Conclusion: Diabetes induced an endothelium dysfunction. Nevertheless, our intense physical training was not effective to restore the aorta endothelial function.

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Figures

Figure 1
Figure 1
Concentration dependent constriction of phenylephrine (%). Concentration dependent contraction of phenylephrine (PE) on thoracic aorta rings obtained from sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD) groups. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. ‡ Significantly different from sedentary diabetic rats (p<0.05). Significantly different from trained diabetic rats (p<0.05).
Figure 2
Figure 2
Concentration dependent relaxation of acetylcholine (%). Concentration dependent relaxation of acetylcholine (ACh) on thoracic aorta rings obtained from sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. * Significantly different from sedentary control rats (p<0.05), † significantly different from trained control rats.
Figure 3
Figure 3
Concentration dependent relaxation ADPβS (%). Concentration dependent relaxation of ADPβS on thoracic aorta rings obtained from sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. * significantly different from sedentary control rats (p<0.05), † significantly different from trained control rats.
Figure 4
Figure 4
(a) ACh-induced relaxation with and without LNAME in sedentary and trained control. Concentration dependent relaxation of acetylcholine (ACh) with and without LNAME on thoracic aorta rings obtained from sedentary control (SC), trained control (TC) group precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. * significantly different from sedentary control rats (p<0.05), † significantly different from trained control rats. (b) ACh-induced relaxation with and without LNAME in sedentary and trained STZ-diabetic rats. Concentration dependent relaxation of acetylcholine (ACh) with and without LNAME on thoracic aorta rings obtained from sedentary diabetic (SD) and trained diabetic (TD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. ‡ Significantly different from sedentary diabetic rats (p<0.05). Significantly different from trained diabetic rats (p<0.05).
Figure 5
Figure 5
ADPβS-induced relaxation with and without LNAME. Concentration dependent relaxation of ADPβS with and without LNAME on thoracic aorta rings obtained from sedentary control (SC) and sedentary diabetic (SD) groups precontracted with PE. Tension is expressed as % relaxation on initial contraction with PE. Values are expressed as mean ±SEM. *** = p<0.001 significantly different from (SC). ‡ ‡ ‡ = p<0.001 significantly different from (SD), groups.
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