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. 2013 Apr;144(4):691-3.
doi: 10.1053/j.gastro.2013.01.066. Epub 2013 Feb 8.

Biologic tissue sampling with untethered microgrippers

Evin Gultepe  1 , Sumitaka Yamanaka, Kate E Laflin, Sachin Kadam, Yoosun Shim, Alexandru V Olaru, Berkeley Limketkai, Mouen A Khashab, Anthony N Kalloo, David H Gracias, Florin M Selaru
Affiliations

Biologic tissue sampling with untethered microgrippers

Evin Gultepe et al. Gastroenterology. 2013 Apr.
No abstract available

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Figures

Figure 1
Figure 1. Untethered, thermo-sensitive μ-grippers for tissue excision
(A–C) Bright field microscopy images of μ-grippers in an (A) open and (B) closed state; the scale bars represent 200 μm. (C) The image showing that the μ-grippers used in our biopsy experiments were over ten times smaller than the current biopsy forceps; the scale bar represents 1mm. (D–F) Endoscopic images of deployment and retrieval of the μ-grippers in an ex vivo porcine model. (D) μ-grippers covering the colon surface, the scale bar represents 2 mm. (E) Close-up image of μ-grippers closing on the colon wall. (F) Retrieval of the μ-gripperswith a magnetic catheter.
Figure 2
Figure 2. PCR results of the excised tissue and histologic analysis from an in vivo biopsy with μ-grippers
(A) cDNA (β-actin:164 bp, CPA:62 bp and IL-6:119 bp) and (B) genomic DNA (ITGB5:1300 bp, MuC4 and KLRN: 500–600 bp) from the tissue obtained with μ-grippers (G) compared to the negative control (N). (C) H&E stained section of cells retrieved with the μ-grippers from porcine esophagus in an in vivo operation. The image shows viable epithelial cells with clear, abundant cytoplasm, consistent with glycogenated cells originating from the superficial epithelial layer of the esophagus. The black arrows point to junctions between intercellular membranes. The nuclei of these cells are clearly stained and the cells appear healthy, with large cytoplasm to nucleus ratios. Also seen and highlighted in the yellow dotted circle is a separate group of cells with pyknotic nuclei, small cytoplasm-nucleus ratio and deeply stained keratin, indicative of desquamated cells. These cells are probably obtained from the mucus layer overlying the esophageal mucosa.
See this image and copyright information in PMC

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