Non contiguous-finished genome sequence and description of Bacillus timonensis sp. nov

Abstract

Bacillus timonensis strain MM10403188(T) sp. nov. is the type strain of a proposed new species within the genus Bacillus. This strain, whose genome is described here, was isolated from the fecal flora of a healthy patient. B. timonensis is an aerobic Gram-negative rod shaped bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,632,049 bp long genome (1 chromosome but no plasmid) contains 4,610 protein-coding and 74 RNA genes, including 5 rRNA genes.

Keywords: Bacillus timonensis; genome.

Figure 1

Phylogenetic tree highlighting the position of Bacillus timonensis strain MM10403188^T relative to other type strains within the Bacillus genus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum-likelihood method within the MEGA software. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 500 times to generate a majority consensus tree. Clostridium botulinum was used as an outgroup. The scale bar represents a 2% nucleotide sequence divergence.

Figure 2

Gram staining of B. timonensis strain MM10403188^T

Figure 3

Transmission electron microscopy of B. timonensis strain MM10403188^T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 900 nm.

Figure 4

Reference mass spectrum from B. timonensis strain MM10403188^T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.

Figure 5

Graphical circular map of the chromosome. From outside to the center: Genes on the forward strand (colored by COG categories), genes on the reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red), GC content, and GC skew.