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. 2010 Jan;2(1):63-6.

Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection

Hossein Yousofi Darani  1 , Firuzeh Ahmadi, Nozhat Zabardast, Hossein Ali Yousefi, Hedayat Shirzad
Affiliations

Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection

Hossein Yousofi Darani et al. Avicenna J Med Biotechnol. 2010 Jan.

Abstract

Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and latex agglutination tests. Sensitivity and specificity of latex test was determined considering culture as golden standard. Sensitivity and specificity of latex agglutination test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of latex agglutination test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the latex agglutination test developed in this study, further work is recommended to improve the test.

Keywords: Latex agglutination test; Sensitivity; Specificity; Trichomonas vaginalis.

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Figures

Figure 1
Figure 1
Immunoelectrophoresis of Trichomonas vaginalis sonicated antigen (wells) probed with rabbit antisera raised against parasite (troughs)
Figure 2
Figure 2
SDS-PAGE of whole rabbit serum in comparison with purified immunoglobulin
Figure 3
Figure 3
Latex agglutination of Trichomonas vaginalis harvested from culture medium and ploysteren coated purified antibody in comparison with negative control
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