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. 2013 Feb 14;4(2):e495.
doi: 10.1038/cddis.2013.8.

Detection of soluble BAG3 and anti-BAG3 antibodies in patients with chronic heart failure

M De Marco, A Falco, A Basile, A Rosati, M Festa, M d'Avenia, M Pascale, F Dal Piaz, R Bisogni, D Barcaroli, G Coppola, F Piscione, A Gigantino, R Citro, R De Rosa, G Vitulano, N Virtuoso, F Manganelli, E Palermo, F Siano, G Rosato, M Hahne, C Tiberti, V De Laurenzi, M C Turco

Detection of soluble BAG3 and anti-BAG3 antibodies in patients with chronic heart failure

M De Marco et al. Cell Death Dis. .
No abstract available

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Figures

Figure 1
Figure 1
(a) Detection of BAG3 protein in supernatants from cultured cardiomyocytes. Human (HCMa) and rat (H9c2) cardiomyocytes, at 80% confluence, were incubated with or without 10% FBS for 16 h at 37 °C in a 5% CO2 atmosphere. Supernatants were dialyzed in a buffer containing 50 mmol/l NaCl and 0.05% IGEPAL, lyophilized, resuspended in 1 ml of RIPA buffer (50 mmol/l Tris HCl pH 7.6, 150 mmol/l sodium chloride, 2 mmol/l sodium orthovanadate, 4 mmol/l EDTA, 10 mmol/l sodium pyrophosphate, 1% NP-40, 0.1% sodium deoxycholate) and analyzed with anti-BAG3 or anti-GAPDH antibodies by western blotting. (b) Mass analysis of BAG3 detected in the sera from two patients affected by CHF. The sera were analyzed with the anti-BAG3 polyclonal antibody TOS-2 in western blotting. (c) ELISA test for detection of anti-BAG3 antibodies in chronic HF patients. Serum samples from 52 CHF patients (EF<45%) and from 84 healthy donors were analyzed for the presence of anti-BAG3 antibodies by ELISA. Results are plotted as arbitrary units (A.U.). Bars in the dot plot depict the median value obtained in the analyzed groups
See this image and copyright information in PMC

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