A DNA-binding protein is required for termination of transcription by RNA polymerase I in Xenopus laevis
- PMID: 2342463
- PMCID: PMC360640
- DOI: 10.1128/mcb.10.6.2793-2800.1990
A DNA-binding protein is required for termination of transcription by RNA polymerase I in Xenopus laevis
Abstract
We describe a partially fractionated in vitro transcription system from Xenopus laevis for the assay of transcription termination by RNA polymerase I. Termination in vitro was found to require a specific terminator sequence in the DNA and a DNA-binding protein fraction that produces a footprint over the terminator sequence.
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