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. 2013 Aug;27(8):1786-9.
doi: 10.1038/leu.2013.57. Epub 2013 Feb 21.

Telomere length and telomerase complex mutations in pediatric acute myeloid leukemia

A M Aalbers, R T Calado, N S Young, C M Zwaan, C Wu, S Kajigaya, E A Coenen, A Baruchel, K Geleijns, V de Haas, G J L Kaspers, T W Kuijpers, D Reinhardt, J Trka, M Zimmermann, R Pieters, V H J van der Velden, M M van den Heuvel-Eibrink

Telomere length and telomerase complex mutations in pediatric acute myeloid leukemia

A M Aalbers et al. Leukemia. 2013 Aug.
No abstract available

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Conflict of interest statement

Disclosure of conflicts of interest

The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1. Leukemic cell telomere length and telomere length in biological subgroups
(A) Telomere length, expressed as telomere to single copy gene ratio (T/S), was determined in leukemic cells derived from 167 pediatric AML patients at diagnosis and total leukocytes derived from 298 healthy NIH blood bank donors, and expressed as a function of age (years). Telomere length in leukemic cells is not associated with age (slope −0.0043 ± 0.0045, r2 0.005, P=0.349), whereas telomere length in total leukocytes decreases as a function of age. The coefficient of variation (CV) in 51 pediatric AML samples that were measured in two independent runs, calculated as mean of the standard deviation (SD) per repeated measurement divided by the mean of all measurements, was 12%. (B) Telomere length by FAB type. Telomere length was not associated with FAB type (median T/S ratio M0: 0.77; M1: 0.71; M2 0.71; M3: 0.62: M4: 0.63; M5: 0.59; M6: 1.14; M7: 1.32; median T/S ratio of complete cohort: 0.65). FAB M1-2 (n=1; T/S ratio 0.38) and unknown FAB types (n=5; T/S ratio 1.09) are not shown. (C) Telomere length by cytogenetic subgroup. Telomere length was not associated with cytogenetic subgroup (median T/S ratio MLL rearranged cases: 0.63; AML-ETO/inv(16): 0.67; t(15;17): 0.61; NK: 0.61; other: 0.75; median T/S ratio of complete cohort: 0.65). (D) Telomere length by molecular subgroup. Patients carrying more than one of the screened mutations are excluded; patients carrying both FLT3/ITD and a WT1 mutation are only included in the WT1 and FLT3/ITD group. Patients carrying a FLT3/TKD mutation are excluded. Telomere length was associated with molecular subgroup (median T/S ratio CEBPA double mutants: 0.85, range: 0.56–1.5; NPM1 mutants: 0.85, range, 0.46–1.0; WT1 mutants: 0.76, range: 0.61–0.79; FLT3/ITD: 0.57, range: 0.28–0.84; WT1 and FLT3/ITD: 0.57, range, 0.37–0.75; wild-type (not carrying any of the former aberrations): 0.67, range: 0.43–0.80). (E) Telomere length by number of cytogenetic aberrations. Nineteen patients, for whom complete karyotypes were not available, are excluded. Telomere length was not associated with the number of cytogenetic aberrations (median T/S ratio 0 aberrations: 0.63; 1 aberration: 0.62; 2 aberrations: 0.64; ≥3 aberrations: 0.76).
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References

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