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. 2013 Feb 20;5(2):594-607.
doi: 10.3390/nu5020594.

Distribution of selenium and oxidative stress in breast tumor-bearing mice

Chih-Hung Guo  1 , Simon Hsia, Pei-Chung Chen
Affiliations

Distribution of selenium and oxidative stress in breast tumor-bearing mice

Chih-Hung Guo et al. Nutrients. .

Abstract

The present study investigated the effects of breast tumors on the blood and tissue distribution of essential trace mineral selenium (Se), and oxidative stress status of mice. Female 10-week-old BALB/cByJNarl mice were randomly assigned into control (CNL) and breast tumor-bearing (TB) groups. TB mice were injected subcutaneously into the right hind thigh with 5 × 10(6) EMT6 mouse mammary tumor cells. After 22 days, we measured Se concentrations, Se-dependent glutathione peroxidase (GPx) activities, and malondialdehyde (MDA) products (indicator of oxidative stress) in plasma, various tissues, and plasma vascular endothelial growth factor (VEGF) concentrations. There were no significant differences in body weights and daily intake between both groups. Compared with the CNL group, TB mice have decreases in plasma Se concentrations and GPx activities, as well as higher plasma VEGF and MDA concentrations. Plasma Se concentrations were also negatively correlated with plasma MDA and VEGF concentrations. Furthermore, tissue Se concentrations and GPx activities in TB animals were lower; whereas the MDA concentrations higher in various tissues including liver, kidney, brain, lung, spleen, and thymic tissues. In conclusion, disruption of Se homeostasis critically reflects oxidative stress in target tissues, thus may increase the risk for progression of breast cancer and metastasis.

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Figures

Figure 1
Figure 1
Changes of body weight in mice. CNL = control (n = 10); TB = tumor-bearing (n = 10). No significant differences were found between CNL and TB groups (p > 0.05).
Figure 2
Figure 2
Plasma (A) Se, (B) GPx activity, (C) MDA, and (D) VEGF concentrations in mice. Bars are mean (SD) or median (IQR). Values above the box plots are outliers. ** p < 0.05 represents comparison with CNL group observation. Se = selenium; GPx = glutathione peroxidase; MDA = malondialdehyde; and VEGF = vascular endothelial growth factor.
Figure 3
Figure 3
Relationships of the plasma variables in TB mice. Plasma Se concentrations were correlated with (A) MDA and (B) VEGF concentrations; Plasma VEGF concentrations were correlated with (C) Px activity and (D) MDA products. Se = selenium; GPx = glutathione peroxidase; MDA = malondialdehyde; and VEGF = vascular endothelial growth factor.
Figure 4
Figure 4
Tissue Se concentrations in (A) liver, (B) kidney, (C) brain, (D) lung, (E) thymus, and (F) spleen. Bars are mean (SD) or median (IQR). Values above the box plots are outliers. ** p < 0.05 represents comparison with CNL group observation. Se = selenium.
Figure 5
Figure 5
Tissue MDA products in (A) liver, (B) kidney, (C) brain, (D) lung, (E) thymus, and (F) spleen. Bars are mean (SD) or median (IQR). Values above the box plots are outliers. ** p < 0.05 represents comparison with CNL group observation. MDA = malondialdehyde.
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