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Comment
. 2013 Mar;62(3):679-81.
doi: 10.2337/db12-1325.

Diabetic foot ulcer microbiome: one small step for molecular microbiology . . . One giant leap for understanding diabetic foot ulcers?

Benjamin A Lipsky  1 , Jean-Louis Richard, Jean-Philippe Lavigne
Affiliations
Comment

Diabetic foot ulcer microbiome: one small step for molecular microbiology . . . One giant leap for understanding diabetic foot ulcers?

Benjamin A Lipsky et al. Diabetes. 2013 Mar.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Steps for analyzing the microbiome of a DFU (adapted from England R, Pettersson M. Pyro Q-CpG: quantitative analysis of methylation in multiple CpG sites by Pyrosequencing. Nat Methods 2005;2:i–ii). After wound sampling, extraction is performed to obtain bacterial/fungal DNA and/or viral RNA. Specific genetic targets of hypervariable regions within bacterial 16S rRNA genes are amplified by the polymerase chain reaction (PCR) and subjected to DNA pyrosequencing. Sequencing by synthesis occurs by a DNA polymerase–driven generation of inorganic pyrophosphate (PPi) with the formation of ATP and ATP-dependent conversion of luciferin to oxyluciferin. The generation of oxyluciferin causes the emission of light pulses, and the amplitude of each signal is directly related to the presence of one or more nucleosides. The different bacteria can be identified by the effective combination of conserved primer-binding sites and intervening variable sequences that facilitate genus and species identification (software Blastn/tBlastn, http://blast.ncbi.nlm.nih.gov/). The identification of all the bacterial genomes amplified determines the composition of DFU flora (microbiome).
See this image and copyright information in PMC

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References

    1. Lipsky BA, Jeffcoate W, Peters EJG, et al. Expert opinion on the management of infections in the diabetic foot. From the International Working Group on the Diabetic Foot. Diabetes Metab Res Rev 2012;28(Suppl. 1):163–178 - PubMed
    1. Lipsky BA, Berendt AR, Cornia PB, et al. 2012 Infectious Diseases Society of America clinical practice guideline for the diagnosis and treatment of diabetic foot infections. Clin Infect Dis 2012;54:e132–e173 - PubMed
    1. Richard JL, Lavigne JP, Sotto A. Diabetes and foot infection: more than double trouble. Diabetes Metab Res Rev 2012;28(Suppl. 1):46–53 - PubMed
    1. Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 2001;14:244–269 - PMC - PubMed
    1. Gardner SE, Hills SL, Franz RA. Clinical signs of infection in diabetic foot ulcers with high microbial load. Biol Res Nurs 2009;11:119–128 - PMC - PubMed