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. 2013 Jan;36(1):85-8.
Epub 2013 Jan 1.

Detection of Simkania negevensis in cell culture by using a monoclonal antibody

Manuela Donati  1 , Maria Di Paolo, Simone Avanzi, Antonietta Di Francesco, Natascia Fiani, Alison Favaroni, Martina Caracciolo, Roberto Cevenini
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  • PMID: 23435820
Free article

Detection of Simkania negevensis in cell culture by using a monoclonal antibody

Manuela Donati et al. New Microbiol. 2013 Jan.
Free article

Abstract

In the present study, a monoclonal antibody (mAb), D5-14, raised in our laboratory against Chlamydia trachomatis LGV2 serotype, stained Simkania negevensis inclusions in S. negevensis-infected cells by using the immunofluorescence test. D5-14 mAb, reacting in immunoblot with an approximately 64-66-kDa protein of C. trachomatis LGV2 serotype, recognized a protein with the same molecular mass when tested with S. negevensis elementary bodies.

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