Effect of direct-current stimulation on the growth plate. In vivo study with rabbits

Abstract

Electrical stimulation of 8 microA direct current was applied to the femoral distal growth plate of young rabbits for periods of 1, 2, and 3 weeks to examine the effect on bone growth. The results were analyzed using X-ray measurement, histological observation including tetracycline labeling, and glycosaminoglycans analysis. No obvious differences between the stimulated side and the control side were observed in the morphological and biochemical studies, but characteristic thickening of the growth plate caused by the accumulation of hypertrophic cells was found in the group stimulated for 2 weeks. Electricity affects the cell behavior of the growth plate, but it is necessary to find more appropriate conditions to stimulate obvious bone growth.