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Comment
. 2013 Feb 19;104(4):741-3.
doi: 10.1016/j.bpj.2013.01.011.

Superresolving dendritic spines

Leslie M Loew  1 , Stefan W Hell
Affiliations
Comment

Superresolving dendritic spines

Leslie M Loew et al. Biophys J. .
No abstract available

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Figures

Figure 1
Figure 1
Structure and microscopy of dendritic spines. Dendritic spines consist of bulbous heads connected to the dendritic shaft with a thin neck depicted here with a 50–150-nm diameter. (Left) An approximate two-photon excitation region (red) rendered by a near-infrared focused beam in the specimen plane. (Right spine) Doughnut-shaped area (yellow) indicates the focal STED beam overlapping the two-photon excitation beam to counteract two-photon excitation throughout the focal region, but not at the doughnut center where fluorescent molecules (circles) are still allowed to fluoresce. (Green) Fluorescent molecules; (black) molecules silenced by STED. When scanning the standard two-photon excitation beam across the spine shaft (left), the intensity profile is substantially larger than the actual width of the shaft. In contrast, the combined two-photon STED modality provides improved resolution, rendering a more faithful width of the spine necks upon scanning, as described by the new articles in this issue (1,2).
See this image and copyright information in PMC

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References

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