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Comment
. 2013 Feb 19;104(4):741-3.
doi: 10.1016/j.bpj.2013.01.011.

Superresolving dendritic spines

Leslie M Loew  1 , Stefan W Hell
Affiliations
Comment

Superresolving dendritic spines

Leslie M Loew et al. Biophys J. .
No abstract available

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Figures

Figure 1
Figure 1
Structure and microscopy of dendritic spines. Dendritic spines consist of bulbous heads connected to the dendritic shaft with a thin neck depicted here with a 50–150-nm diameter. (Left) An approximate two-photon excitation region (red) rendered by a near-infrared focused beam in the specimen plane. (Right spine) Doughnut-shaped area (yellow) indicates the focal STED beam overlapping the two-photon excitation beam to counteract two-photon excitation throughout the focal region, but not at the doughnut center where fluorescent molecules (circles) are still allowed to fluoresce. (Green) Fluorescent molecules; (black) molecules silenced by STED. When scanning the standard two-photon excitation beam across the spine shaft (left), the intensity profile is substantially larger than the actual width of the shaft. In contrast, the combined two-photon STED modality provides improved resolution, rendering a more faithful width of the spine necks upon scanning, as described by the new articles in this issue (1,2).
See this image and copyright information in PMC

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References

    1. Bethge P., Chereau R., Nägerl V. Two-photon excitation STED microscopy in two colors in acute brain slices. Biophys. J. 2013;104:778–785. - PMC - PubMed
    1. Takasaki K., Ding J., Sabatini B. Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy. Biophys. J. 2013;104:770–777. - PMC - PubMed
    1. Hell S.W. Microscopy and its focal switch. Nat. Methods. 2009;6:24–32. - PubMed
    1. Nägerl U.V., Willig K.I., Bonhoeffer T. Live-cell imaging of dendritic spines by STED microscopy. Proc. Natl. Acad. Sci. USA. 2008;105:18982–18987. - PMC - PubMed
    1. Ding J.B., Takasaki K.T., Sabatini B.L. Supraresolution imaging in brain slices using stimulated-emission depletion two-photon laser scanning microscopy. Neuron. 2009;63:429–437. - PMC - PubMed

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