Repeated evolution of salt-tolerance in grasses

Abstract

The amount of salt-affected agricultural land is increasing globally, so new crop varieties are needed that can grow in salt-affected soils. Despite concerted effort to develop salt-tolerant cereal crops, few commercially viable salt-tolerant crops have been released. This is puzzling, given the number of naturally salt-tolerant grass species. To better understand why salt-tolerance occurs naturally but is difficult to breed into crop species, we take a novel, biodiversity-based approach to its study, examining the evolutionary lability of salt-tolerance across the grass family. We analyse the phylogenetic distribution of naturally salt-tolerant species on a phylogeny of 2684 grasses, and find that salt-tolerance has evolved over 70 times, in a wide range of grass lineages. These results are confirmed by repeating the analysis at genus level on a phylogeny of over 800 grass genera. While salt-tolerance evolves surprisingly often, we find that its evolution does not often give rise to a large clade of salt-tolerant species. These results suggest that salt-tolerance is an evolutionarily labile trait in grasses.

Figure 1.

Distribution of 200 salt-tolerant species (circles at tips) on a phylogeny of grasses, modified from Edwards et al. [11]. We reconstructed 76 origins of salt-tolerance (squares on internal nodes) using the best-fitting likelihood model. Most origins occur close to the tips of the phylogeny, with a mean of 2.6 halophytes per origin.