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. 2012 Mar;52(1):104-8.
doi: 10.1007/s12088-011-0227-x. Epub 2011 Sep 14.

Isolation and Characterization of a Bacteriocin-Like Substance Produced by Geobacillus toebii Strain HBB-247

Gamze Başbülbül Özdemir  1 , Haci Halil Biyik
Affiliations

Isolation and Characterization of a Bacteriocin-Like Substance Produced by Geobacillus toebii Strain HBB-247

Gamze Başbülbül Özdemir et al. Indian J Microbiol. 2012 Mar.

Abstract

A total of 201 thermophilic bacteria isolated from various thermal spring, mud and soil were tested for their antibacterial activity. Among the mostly active isolates, Geobacillus toebii HBB-247 was further examined. Bacteriocin-like inhibitory substance (BLIS) produced by strain HBB-247 was found to be stable up to 60°C, sensitive to proteolytic enzymes and effective against Enterococcus faecalis, Listeria sp., E. avium, Clostridium pasteurianum, Cellulomonas fimi and some thermophilic strains isolated and identified in this study. As a result of Tricine-SDS-PAGE molecular weight of BLIS was estimated about 38 kDa. Production studies showed that G. toebii HBB-247 starts to produce antibacterial substance at early logarithmic phase of growth and maximum production was detected at the end of the logarithmic phase.

Keywords: Antibacterial; BLIS; Characterization; Geobacillus; Isolation.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree showing the position of strain HBB-247 within the branches of the genus Geobacillus. Bar indicates 0.005 substitutions per nucleotide position. Bootstrap values (%) are based on 1,000 replicates and branches with 50% and more bootstrap support are shown
Fig. 2
Fig. 2
Tricine-SDS-PAGE. Left to right molecular weight marker, protein profile of the ammonium sulphate precipitated culture supernatant of strain HBB-247 and direct overlay of gel with G. stearothermophilus DSMZ 22 indicating inhibition zone
Fig. 3
Fig. 3
Production of BLIS during growth of G. toebii HBB-247. Symbols: filled square absorbance, filled diamond antibacterial activity of BLIS (AU/ml)
See this image and copyright information in PMC

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