doi: 10.1099/00221287-86-2-294.
The structural gene for NADP L-glutamate dehydrogenase in Aspergillus nidulans
- PMID: 234511
- DOI: 10.1099/00221287-86-2-294
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The structural gene for NADP L-glutamate dehydrogenase in Aspergillus nidulans
J Gen Microbiol.
1975 Feb.
Abstract
A total of 41 mutants lacking NADP L-glutamate dehydrogenase (NADP-GDH) activity have been studied. All the mutations were located at the gdhA locus within 0-1% recombination of gdhAI. Two mutants, gdhAI and gdhA2, out of five examined, produced cross-reacting material which neutralized NADP-GDH anti-serum. The mutant gdhA9 has altered Km values for all five substrates: ammonium, alpha-ketoglutarate, l-glutamate, NADPH and NADP. The mutant gdhA20 had temperature-sensitive growth, abnormal ammonium-regulation characteristics and thermolabile NADP-GDH activity. These results show that gdhA is the structural gene for NADP-GDH.
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