Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Mar 1:13:51.
doi: 10.1186/1472-6882-13-51.

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

Fifa T D Bothon  1 , Eric Debiton, Felicien Avlessi, Christiane Forestier, Jean-Claude Teulade, Dominique K C Sohounhloue
Affiliations

In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

Fifa T D Bothon et al. BMC Complement Altern Med. .

Abstract

Background: Extracts from Polygonum senegalensis (Polygonaceae) and Pseudocedrela kotschyi (Meliaceae) are two important traditionally used medicinal plants in rural Benin to treat many diseases and notably type 2 diabetes. The aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those plants extract: Polygonum senegalensis leaves, and Pseudocedrela kotschyi root.

Methods: Hydro-alcoholic (50%) extracts were analyzed for their phytochemical content and tested for their inhibition potency on α-glucosidase from Saccharomyces cerevisiae. Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay. Finally, the antibacterial activity was evaluated using MIC determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast Candida albicans.

Results: Each extract presented significant α-glucosidase inhibition and antioxidant activities. Polygonum senegalensis leaf extracts were the most active in each in vitro assay with an IC50 = 1.5 μg/ml for α-glucosidase inhibition and an IC50 = 6.8 μg/ml for DPPH scavenging, - 4.5 μmol Fe II/g of dry matter - 9366 μmol Trolox / g DW - for FRAP and ORAC values, respectively. IC50 = 2.3 μg GA / ml for DCFH-DA assay. Concerning its antibacterial activity, a growth inhibitory effect was observed only against three Gram negative bacilli: B. subtilis, E. faecalis, S. aureus and the yeast C. albicans at high concentration.

Conclusion: The results showed that the semi alcoholic extract of the two studied plants possess α-glucosidase inhibitory activity, antioxidant potency, and low antibacterial effect.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The α-glucosidase inhibitory activity of the extracts of the two plants at increasing concentrations.
Figure 2
Figure 2
Antioxidant activity of extracts. A: 2, 2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging, B: Ferric Reducing Antioxidant Power (FRAP) values, C: Oxygen Radical Absorbance Capacity (ORAC), D: Antioxidant effects of extracts using the DCFH-DA cell-based assay.
See this image and copyright information in PMC

References

    1. Lee J, Koo N, Min DB. Reactive oxygen species, aging, and antioxidative nutraceuticals. Compr Rev Food Sci F. 2004;3:22–33. - PubMed
    1. World Health Organization. Who launches the first global strategy on traditional medicine. Geneva; Press release WHO 2002/38. http://www.who.int/mediacentre/news/releases/release38/en/
    1. Udeme OG, Owunari AG. Effect of extract of Pseudocedrela kotschyi on blood glucose concentration of alloxan induced diabetic albino rats. East J Med. 2009;14:17–19.
    1. Asase A, Kokubun T, Grayer RJ, Kite G, Simmonds MS, Oteng-Yeboah AA, Odamtten GT. Chemical constituents and antimicrobial activity of medicinal plants from Ghana: Cassia sieberiana, Haematostaphis barteri, Mitragyna inermis and Pseudocedrela kotschyi. Phytother Res. 2008;22:1013–1016. doi: 10.1002/ptr.2392. - DOI - PubMed
    1. de Sousa E, Leila Zanatta IS, Creczynski-Pasa TB, Pizzolatti MG, Szpoganicz B, Barreto Silva FRM. Hypoglycemic Effect and Antioxidant Potential of Kaempferol-3,7-O-(r)-dirhamnoside from Bauhinia forficata Leave. J Nat Prod. 2004;6:829–832. - PubMed

Publication types

MeSH terms

LinkOut - more resources