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. 2013 Mar 15;15(6):1330-3.
doi: 10.1021/ol400268t. Epub 2013 Mar 5.

Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R G Pitter  1 , Jens Wigenius, Adrienne S Brown, James D Baker, Fredrik Westerlund, James N Wilson
Affiliations

Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R G Pitter et al. Org Lett. .

Abstract

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

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